megatomi.com - An Overview
megatomi.com - An Overview
Blog Article
Promptly receive sections as slender or thick as your application needs, devoid of worrying about shedding or distorting valuable tissue samples.
Megatome is often a vibrating microtome meant to section a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform area profile, and negligible tissue damage and information loss.
Antibodies may well acquire months to diffuse by just a few millimeters of tissue, that has a steep labeling gradient from floor to Main.
Working the CLI directly from a Gradle job isn't presently supported. A distribution must be created by using gradlew :j2d-cli:distZip to produce a zip file made up of anything necessary to run.
SE makes use of a rotational electric field to disperse highly electromobile molecules (which include antibodies or surfactant micelles) all through a porous sample without detrimental electrically charged structures within the tissue. This allows two-4 day clearing of intact organs,
task to make an aggregated Javadoc. In this occasion, javadocTask needs to be set to allJavadoc to ensure that the proper documentation is developed before building the docset.
Megatome is usually a novel microtome that enables for high-precision sectioning of an array of tissue samples – from organoids, to megatomi.com arrays of animal organs, to intact human Mind hemispheres – with minimum tissue destruction and data loss.
eFLASH is often a fast tissue labeling system that permits for uniform whole-organ staining in twenty rounds of labeling.
The SmartSPIM light sheet microscope supplies unparalleled resolution and acquisition speed through intact tissue volumes, whilst its modular style and design enables you to enhance and customize particular person components.
Docset creation involves at minimal two selections: the name with the docset and the location on the Javadoc data files to include during the docset.
This can make a docset named Sample in the current directory. Docset generation might be personalized with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the knowledge reduction from PFA preservation, preserving specimens for numerous rounds of processing.
Leverage the Obvious+ tissue clearing process, along with eFLASH and patented stochastic electrotransport systems, to speedily distinct and label complete organs. Critical highlights and options include:
Our novel SHIELD tissue preservation approach varieties intramolecular bonds working with polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.